| 质粒名称: | pHAGE PGK-GFP-IRES-LUC-W |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46793 |
| 存储实验室: | Darrell Kotton |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE |
| 载体出品公司: | Richard Mulligan |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4874 bp |
| 完整质粒大小: | 9272 bp |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PGK,PGK promoter |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 465 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE PGK-GFP-IRES-LUC-W was a gift from Darrell Kotton (Addgene plasmid # 46793 ; http://n2t.net/addgene:46793 ; RRID:Addgene_46793) |
| 参考文献部分: | Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, Kotton DN. J Clin Invest. 2010 Jan . 120(1):379-89. 10.1172/JCI36666 PubMed 20038801 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE PGK-GFP-IRES-LUC-W 9272 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 2nd generation lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS pHAGE PGK-GFP-IRES-LUC-W
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9272)
AUTHORS Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH,
Mulligan RC, Amar S, Suki B, Kotton DN
TITLE Amelioration of emphysema in mice through lentiviral transduction of
long-lived pulmonary alveolar macrophages.
JOURNAL J Clin Invest. 2010 Jan . 120(1):379-89.
PUBMED 20038801
REFERENCE 2 (bases 1 to 9272)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9272
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind complement(2261..2284)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2611..2630
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 2686..2695
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2692..3411
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2737..2758)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2998..3017)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3345..3366
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3415..3982
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3432..3984
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3576..3593)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3803..3822
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 3996..5648
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(4062..4080)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
misc_feature 5662..6250
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5715..5735)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6133..6144)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6325..6558
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6677..6695)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6763..6867
/gene="bla"
/label=AmpR promoter
CDS 6868..7728
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7086..7105)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7899..8487
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8388..8407
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8641..8658
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 8750..8769
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 8898..8919
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8934..8964
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8972..8988
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8977..8999
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8996..9012
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8996..9012
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9062..9081
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcatt
2221 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
2281 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
2341 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
2401 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
2461 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
2521 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg
2581 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
2641 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcggccgc catggtgagc
2701 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
2761 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
2821 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
2881 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
2941 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
3001 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
3061 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
3121 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
3181 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
3241 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
3301 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
3361 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aggatccctc
3421 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
3481 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
3541 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
3601 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
3661 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
3721 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
3781 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
3841 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
3901 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
3961 cctttgaaaa acacgatgat aatatggcca cacatatgga agacgccaaa aacataaaga
4021 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
4081 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
4141 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
4201 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
4261 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
4321 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
4381 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
4441 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
4501 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
4561 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
4621 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
4681 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
4741 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
4801 aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
4861 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
4921 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
4981 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
5041 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
5101 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
5161 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
5221 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
5281 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
5341 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg
5401 tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
5461 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
5521 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
5581 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
5641 ccgtgtaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
5701 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
5761 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
5821 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
5881 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
5941 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
6001 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
6061 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
6121 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
6181 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
6241 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
6301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
6361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
6541 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
6601 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
6661 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
6721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
6781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
6841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
6901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
6961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
7021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
7081 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
7141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
7201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
7261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
7321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
7381 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
7441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
7501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
7561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
7621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
7681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
7741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
7801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
7861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
7921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
7981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
8041 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
8101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
8161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
8221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
8281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
8341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
8401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
8461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
8521 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
8581 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
8641 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
8701 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
8761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
8821 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
8881 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
8941 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
9001 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
9061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
9121 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
9181 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
9241 atttgttaaa tatgtactac aaacttagta gt
//
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